Though practces to minimize viral transmission have been used in hemodialysis units for many years, many studies have shown that incidences of viral transmission contnue to occur in hemodialysis centers and the prevalence and seroconversion greatly vary among hemodialysis facilites. More recent studies in some hemodialysis facilites have shown decline in seroconversion, where as the transmission of these diseases contnues to persist within several dialysis units. However, there are no published data on prevalence and seroconversion of these diseases in patents undergoing maintenance hemodialysis in Nepal. So this study was conducted to evaluate the prevalence and seroconversion of hepatts B, hepatts C and HIV infecton among Nepalese hemodialysis populaton. The study was a prospectve hospital based study done in 54 end-stage renal disease (ESRD) patents on maintenance hemodialysis (MHD). Serological tests for HBsAg, ant HCV antbodies and ant HIV-1 & 2 antbodies, using third generaton Enzyme-Linked immunosorbent assay (ELISA) were done. These serological tests were repeated to see any seroconversion afer 18 months. The mean age of the HD patents was 47.52 (±13.54) years. The causes of ESRD were chronic glomerulonephrits (CGN) (40%), diabetes (20%), hypertension (9%) and others (ADPKD, obstructve uropathy and unknown) (31%). The mean HD duraton was 32.32 (± 22.61) months. Seroconversion of HBV, HCV and HIV was nil during 18 months follow up. The zero seroconversion of the mentoned viral diseases in our hemodialysis units might be due to several factors such as strict adherence to standard infecton control practce, vaccinaton of all the patents for hepatts B, segregaton of patents, regular screening of the patents for the viral disease, use of erythropoietn. Though there was no seroconversion of HBV, HCV or HIV in our dialysis populaton, this should not lead to compromise in standard infecton control practce and screening for these potentally lethal complicatons.Key words: Chronic renal failure, hemodialysis, hepatts B and C